MBD5000

GenElute^™ Microbiome DNA Purification Kit

High yield for PCR, sequencing and NGS

• UNSPSC Code: 41105501
• NACRES: NA.52

Properties

• Quality Level: 200
• technique(s): DNA purification: suitable (from stool )
• shipped in: ambient
• storage temp.: room temp

Description

Application

In order to study the role of gut microbiota in human health and disease, accurate and reproducible microbial data must be obtained.The crucial step in this process is to apply an appropriate methodology to extract microbial DNA. Setting up the optimal DNA isolation procedure is critical for robustness and reproducibility of the results. Inaccurate DNA extraction may result in microbial misrepresentation.
The Microbiome DNA Purification Kit provides a convenient and rapid method to isolate high-molecular weight, high-quality and high yield microbial DNA from stool samples. The kit contains DNA-free reagents to assure that no external contamination is present. The kit uses a combination of chemical, mechanical and thermal lysis with a simple and rapid spin column procedure to purify the DNA. The purified DNA is high molecular weight, high quality and is fully compatible with downstream applications, such as PCR and NGS.

application note: Typical DNA recovery is 5–25μg from 0.2 g of human stool or 0.1 g of mouse stool.

Features and Benefits

• Accurate and reproducible results
• Convenient and rapid spin column procedure
• Highly efficient lysis: combination of chemical, mechanical and thermal methods
• High molecular weight, high quality and high yield DNA is obtained
• DNA free reagents to assure no external contamination
• Fully compatible with downstream applications such as PCR, sequencing and NGS

Components

This kit contains reagents sufficient for 50 DNA extractions.
.

• This product is comprised of the following:
• M1 Lysis Buffer MBD5001
• M2 Lysis Boost Buffer MBD5002
• M3 Cleaning Buffer MBD003
• M4 Binding Buffer MBD5004
• M5 Wash Buffer MBD005
• M6 Elution Buffer MBD006
• Columns MBD5007
• Collection Tubes MBD5008
• Lysis Beads MBD5009

See datasheet for more information

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H332,H314,H412
• Precautionary Statements: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• Supplementary Hazards: EUH032
• Storage Class Code: 8A - Combustible corrosive hazardous materials