N5386

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

• Synonym(s): Endonuclease micrococcal, MNase, Micrococcal endonuclease
• CAS Number: 9013-53-0
• Enzyme Commission number: 3.1.31.1 (BRENDA, IUBMB)
• EC Number: 232-748-0
• MDL number: MFCD00131731
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Staphylococcus aureus
• Quality Level: 200
• form: powder
• specific activity: 100-300 units/mg protein
• composition: Protein, ≥40% E1%/280 (Balance primarily sodium acetate)
• technique(s): DNA extraction: suitable; DNA purification: suitable
• suitability: suitable for molecular biology
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Description

Application

Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.

Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves