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Safety Information

N5389

Sigma-Aldrich

Anti-Neurofilament H (200 kDa) Antibody

enhanced validation

mouse monoclonal, NE14

Synonym(s):

NF200 Antibody - Monoclonal Anti-Neurofilament 200 antibody produced in mouse, Neurofilament Antibody, Nf200 Antibody, Monoclonal Anti-Neurofilament heavy chain

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About This Item

MDL number:
MFCD02263444
UNSPSC Code:
12352203
NACRES:
NA.41
conjugate:
unconjugated
application:
ARR
IHC (f)
IHC (p)
WB
clone:
NE14, monoclonal
species reactivity:
pig, mouse, chicken, bovine, guinea pig, rabbit, rat, human
citations:
84
technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
immunohistochemistry (frozen sections): suitable
microarray: suitable
western blot: suitable

Product Name

Monoclonal Anti-Neurofilament 200 antibody produced in mouse, clone NE14, ascites fluid

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NE14, monoclonal

mol wt

antigen apparent mol wt 200 kDa

contains

15 mM sodium azide as preservative

species reactivity

pig, mouse, chicken, bovine, guinea pig, rabbit, rat, human

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
immunohistochemistry (frozen sections): suitable
microarray: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

P12036

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NEFH(4744)

General description

Monoclonal Anti-Neurofilament 200 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The neurofilaments are one of the five major groups of intermediate filaments (IFs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 160 kD, and 200 kD and are named as NEFL (light), NEFM (medium) and NEFH (heavy) respectively. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals.

Immunogen

pig spinal cord.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Neurofilament 200 antibody produced in mouse has been used in
  • immunohistochemistry
  • immunolabeling
  • immunofluorescence

Biochem/physiol Actions

Neurofilament heavy chain (NEFH) gene was recently identified to cause autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc). This gene plays a role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

N5389-.5ML:
N5389-.2ML:
N5389-100UL:
N5389-VAR:
N5389-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000381747
0000381747
0000324047
0000324046
0000284012

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Shaun M Logan et al.
Neuroscience letters, 545, 69-74 (2013-04-30)
Recent studies have shown that ephrin-B2 on sensory afferent fibers from the dorsal root ganglia (DRG) controls transmission of pain sensation to the spinal cord. We examined ephrin-B2 expression in mouse DRG and spinal cord using an ephrin-B2/ß-galactosidase chimeric allele.
Neurofilament proteins are synthesized in nerve endings from squid brain
Crispino M, et al.
Journal of Neurochemistry, 61(3), 1144-1146 (1993)
Victoria A Baronas et al.
Nature communications, 9(1), 4417-4417 (2018-10-26)
Kv1.2 is a prominent voltage-gated potassium channel that influences action potential generation and propagation in the central nervous system. We explored multi-protein complexes containing Kv1.2 using mass spectrometry followed by screening for effects on Kv1.2. We report that Slc7a5, a
Pamela Imperadore et al.
Scientific reports, 7, 46564-46564 (2017-04-21)
Regeneration is a process that restores structure and function of tissues damaged by injury or disease. In mammals complete regeneration is often unsuccessful, while most of the low phyla animals can re-grow many parts of their body after amputation. Cephalopod
Cell cycle kinetics and immunohistochemical characterization of dissociated fetal neocortical cultures: evidence that differentiated neurons have mitotic capacity
Jacobs JS and Miller MW
Dev. Brain Res., 122(1), 67-80 (2000)
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