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Safety Information

N9284

Sigma-Aldrich

Nitroreductase from Escherichia coli

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≥90% (SDS-PAGE), recombinant, expressed in E. coli

Synonym(s):

NTRA

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About This Item

MDL number:
MFCD03457727
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥100 units/mL

mol wt

monomer 24000

greener alternative product characteristics

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Safer Solvents and Auxiliaries
Design for Energy Efficiency
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UniProt accession no.

P38489

greener alternative category

, Re-engineered

shipped in

wet ice

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... nfsB(945483)

Related Categories

General description

Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.

Application

Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.

Biochem/physiol Actions

Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Physical properties

Lyophilized powder containing PBS. Does not contain BSA as excipient

Unit Definition

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

Preparation Note

Produced using animal component-free materials.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

N9284-PH:
N9284-VAR:
N9284-BULK:
N9284-1MG:
I-3627:
N9284-1MG-PW:
N9284PROC:

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Certificates of Analysis (COA)

Lot/Batch Number
0000206122
SLBZ7010
SLBR5273V
011M7677
109K3788

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Generation of Escherichia coli nitroreductase mutants conferring improved cell sensitization to the prodrug CB1954
Grove JI, et al.
Cancer research, 63(17), 5532-5537 (2003)
Mckayla Stevens et al.
Bioorganic & medicinal chemistry, 28(22), 115710-115710 (2020-10-03)
In two previous studies, we identified compound 1 as a moderate GroEL/ES inhibitor with weak to moderate antibacterial activity against Gram-positive and Gram-negative bacteria including Bacillus subtilis, methicillin-resistant Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, and SM101 Escherichia coli (which has
Bharat Bhushan et al.
Biochemical and biophysical research communications, 322(1), 271-276 (2004-08-18)
Previously, we reported that a salicylate 1-monooxygenase from Pseudomonas sp. ATCC 29352 biotransformed CL-20 (2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaaza-isowurtzitane) (C(6)H(6)N(12)O(12)) and produced a key metabolite with mol. wt. 346 Da corresponding to an empirical formula of C(6)H(6)N(10)O(8) which spontaneously decomposed in aqueous medium to
M J Lemmon et al.
Gene therapy, 4(8), 791-796 (1997-08-01)
A fundamental obstacle in gene therapy for cancer treatment is the specific delivery of an anticancer gene product to a solid tumor. Although several strategies exist to control gene expression once a vector is directly introduced into a tumor, as
Chih-Chen Chen et al.
Food chemistry, 135(4), 2708-2713 (2012-09-18)
Nitroreductases (Nrs) play important roles in redox system via NADPH or NADH as a reductant. A TcNr cDNA encoding a putative Nr was cloned from Taiwanofungus camphorata. A 3-D structural model of the TcNr has been created based on the
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