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Key Documents

Safety Information

P1189

Sigma-Aldrich

Polymyxin B

liquid, suitable for cell culture, BioReagent

Synonym(s):

Aerosporin, PMB, PMX, Polymyxin, Polymyxin B

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.76

product name

Polymyxin B ready made solution, Cell culture tested, 17 mg/mL in H2O

Quality Level

200

form

liquid

concentration

17 mg/mL in H2O

storage temp.

2-8°C

General description

Polymyxin B (PMB) is a cyclic cationic lipopeptide antibiotic originally produced by soil bacterium Bacillus polymyxa. Polymyxin B binds specifically to gram negative bacteria by interacting with the anionic lipopolysaccharide (LPS, or endotoxin). Polymyxin B interferes with the outer membrane normal function causing leakage and enhanced uptake of the antibiotic.

Polymyxin B has several applications:
  • It is used to neutralize LPS contamination in vitro and in vivo. Its neutralizing effect is dose-dependent. In vitro experiments showed that PMB inhibits a septic shock by endotoxin inactivation.
  • Cell cultures can be treated with PMB (100U/mL) to avoid early contaminations. PMB is considered a broad spectrum antibiotic and is used to treat infections caused by bacteria resistant to a combination of Penicillin and Streptomycin.
  • PMB is also used as negative and positive control in minimum microbicidal concentration (MIC) assay.

Working concentration:
The suggested concentration of PMB in cell culture mediums is 100U/mL. Therefore, it is recommended to dilute PMB ready made solution 1:1000.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P1189-1ML:
P1189-BULK:
P1189-5ML-PW:
P1189-5ML:
P1189-VAR:
P1189-1ML-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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