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P1944

Sigma-Aldrich

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

Synonym(s):

phenol chloroform RNA extraction

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About This Item

UNSPSC Code:
12190000
NACRES:
NA.52
grade:
for molecular biology
Assay:
≥99.0%
application(s):
life science and biopharma

grade

for molecular biology

Quality Level

200

Assay

≥99.0%

form

liquid

pH

4.3-4.7

application(s)

life science and biopharma

storage temp.

2-8°C

General description

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

Application

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.
  • to isolate RNA from Arabidopsis siliques.
  • to extract proteins and DNA from polysomal and non-polysomal fractions.
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Target Organs

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

175.0 °F - closed cup

Flash Point(C)

79.44 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

PRTR

Class I Designated Chemical Substances

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

P1944-VAR:
P1944-100ML-PW:
P1944-400ML-PW:
P1944-100ML:4548173205519
P1944-BULK:
P1944-400ML:4548173205526

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW7584
MKCV5362
MKCV5361
MKCT1646
MKCT0359

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Exploring translation regulation by global analysis of ribosomal association
Melamed D, et al.
Methods in Enzymology, 48(3), 301-305 (2009)
Molecular approaches for analysis of Drosophila MicroRNAs
Pandey M, et al.
Journal of Separation Science, 169-188 (2020)
Autophagy controls resource allocation and protein storage accumulation in Arabidopsis seeds
Berardino JD, et al.
Journal of Experimental Botany, 69(6), 1403-1414 (2018)
Machine learning-assisted global DNA methylation fingerprint analysis for differentiating early-stage lung cancer from benign lung diseases
Lu D, et al.
Biosensors And Bioelectronics, 235, 115235-115235 (2023)
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
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