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Safety Information

P3738

Sigma-Aldrich

Protein Kinase G Iβ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

Synonym(s):

cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human

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About This Item

Enzyme Commission number:
2.7.11.12 (BRENDA, IUBMB)
MDL number:
MFCD03458230
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))

mol wt

76 kDa (monomer)

UniProt accession no.

Q13976

relevant disease(s)

cancer

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PRKG1(5592)

Application

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

Biochem/physiol Actions

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

Unit Definition

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

Physical form

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

Cartagena Act

Cartagena Act Listed

JAN Code

P3738-VAR:
P3738-5UG-PW:
P3738-BULK:
P3738-5UG:

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Certificates of Analysis (COA)

Lot/Batch Number
020M1326
010M1130
129K1204
119K1477
119K1185

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Atsuko Deguchi et al.
Cancer research, 65(18), 8442-8447 (2005-09-17)
Recent studies indicate that the induction of apoptosis in human colon cancer cells by certain nonsteroidal antiinflammatory drugs involves increased expression of 15-LOX-1 and synthesis of its major product 13-S-hydroxyoctadecadienoic acid (13-S-HODE). Evidence was obtained that this occurs via a
Faranak Fallahian et al.
Cell biochemistry and function, 30(3), 183-190 (2011-11-19)
Activation of protein kinase G (PKG) by cyclic guanosine 3,5-monophosphate (cGMP) has become of considerable interest as a novel molecular approach for the induction of apoptosis in cancer cells. This study was conducted to investigate the role of PKG isoforms
D Pöhler et al.
FEBS letters, 374(3), 419-425 (1995-11-06)
Detailed studies of differences in distinct cGMP kinase isoforms are highly dependent on expression of large amounts of these enzyme isoforms that are not easily purified by conventional methods. Here cGMP-dependent protein kinases, the type I beta soluble form from

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