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R7397

Sigma-Aldrich

ProtectRNA™ RNase Inhibitor 500× Concentrate

RNase inhibitor for in situ hybridization assays

Synonym(s):

Ribonuclease inhibitor ProtectRNA™

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.52

Quality Level

200

form

liquid

storage temp.

2-8°C

General description

Not recommended in systems where other enzymatic activity is required.

ProtectRNA™ ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.

Application

ProtectRNA™ RNase Inhibitor 500× Concentrate has been used in:
  • RNA extraction
  • tissue sectioning and staining for laser capture microscopy
  • the isolation of blood vessels from hippocampi
ProtectRNA™ RNase Inhibitor 500× Concentrate is an RNase inhibitor.
A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.

Features and Benefits

  • The 500× concentrate is economical
  • Eliminates the need for special dishwashing and“after washing” cleaning and treatments.

Quantity

2mL treats 1,000ml of solution.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Legal Information

ProtectRNA is a trademark of Sigma-Aldrich Co. LLC

Pictograms

FlameCorrosion
GHS02,GHS05

Signal Word

Warning

Hazard Statements

H226,H290

Hazard Classifications

Flam. Liq. 3 - Met. Corr. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

140.0 °F - closed cup

Flash Point(C)

60 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

R7397-15ML:
R7397-BULK:
R7397-VAR:
R7397-30ML:4548173951249

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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