SIALICQ

Sialic Acid Quantitation Kit

Sufficient for 25 reactions

• UNSPSC Code: 41116107
• NACRES: NA.32

Properties

• Quality Level: 200
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Sialic Acid Quantitation Kit provides a rapid and accurate determination of total N-acetylneuraminic acid (NANA; sialic acid). NANA is released from glycoconjugates using neuraminidase. The kit uses α(2→3,6,8,9) neuraminidase to cleave all NANA linkages, including α(2→8) and α(2→9) linkages, as well as branched NANA, for the most accurate determination of total sialic acid content.

Application

Contains all reagents to quantitate the amount of N-acetylneuraminic acid, either free, or in glycoproteins, cell surface glycoproteins, polysialic acids and capsular polysaccharides (consisting only of polysialic acid).

Quantity

Sufficient reagents are provided to perform 25 reactions, including control samples. Each reaction will measure 1-200 nmoles of NANA.

Kit Components Only

• N-Acetylneuraminic acid aldolase 25 μL
• N-Acetylneuraminic acid 200 μL
• α(2→3,6,8,9)-Neuraminidase 25 μL
• Fetuin, bovine .5 mg
• Lactic dehydrogenase 25 μL
• β-NADH disodium salt 3 vial(s)
• Tris-HCl, pH 7.5 1 mL
Sialidase buffer 500 μL

See All (8)

Safety Information

• Storage Class Code: 10 - Combustible liquids