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Key Documents

Safety Information

SRP4524

Sigma-Aldrich

BD-3 human

recombinant, expressed in E. coli

Synonym(s):

DEFB103, DEFB3, HBD3, HBP3, HDB-3

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

form

lyophilized

mol wt

~5 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

P81534

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DEFB103A(414325)

General description

β-defensin-3 (BD-3) is an anti-microbial peptide produced by airway epithelial cells, lung-derived epithelial cells and keratinocytes. It has a molecular weight of 5kDa. The gene encoding this protein is localized on human chromosome 8p23.1. Recombinant human β-defensin-3 (BD-3) is an antimicrobial peptide, which belongs to the distinct family of β-defenisns. Recombinant human BD-3 is a 5.1 kDa protein containing 45 amino acid residues.
Chemical structure: peptide

Biochem/physiol Actions

β-defensin-3 (BD-3) associates with bacterial products which reduce the effect of inflammatory cytokine responses. The protein has been shown to stimulate the production of interferon-α and activate monocytes through toll-like receptors 1 and 2. It antagonizes the activation of C-X-C motif chemokine receptor 4 (CXCR4).

Physical form

Sterile filtered and lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in 10 mM Acetic acid to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP4524-20UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jeffrey P Meisch et al.
Inflammatory bowel diseases, 19(5), 942-953 (2013-03-21)
Antimicrobial peptides (AMPs) maintain a sterile environment in intestinal crypts, limiting microbial colonization and invasion. Decreased AMP expression is proposed to increase the risk for inflammatory bowel disease. Expression and function of inducible AMPs, human β-defensin 2 and 3 (hBD-2
Edward J Hollox et al.
Nature genetics, 40(1), 23-25 (2007-12-07)
Psoriasis is a common inflammatory skin disease with a strong genetic component. We analyzed the genomic copy number polymorphism of the beta-defensin region on human chromosome 8 in 179 Dutch individuals with psoriasis and 272 controls and in 319 German
J Harder et al.
The Journal of biological chemistry, 276(8), 5707-5713 (2000-11-22)
The growing public health problem of infections caused by multiresistant Gram-positive bacteria, in particular Staphylococcus aureus, prompted us to screen human epithelia for endogenous S. aureus-killing factors. A novel 5-kDa, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human
Stefanie Scharf et al.
Respiratory research, 11, 93-93 (2010-07-10)
Legionella pneumophila is an important causative agent of severe pneumonia in humans. Human alveolar epithelium and macrophages are effective barriers for inhaled microorganisms and actively participate in the initiation of innate host defense. The beta defensin-3 (hBD-3), an antimicrobial peptide
Poonam Tewary et al.
Journal of immunology (Baltimore, Md. : 1950), 191(2), 865-874 (2013-06-19)
Alarmins are a group of structurally diverse host defense antimicrobial peptides that are important immune activators. In this article, we present a novel role for two potent alarmins, human β-defensin 2 and 3 (HBD2 and 3), in promoting IFN-α production
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