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CFPS700

Sigma-Aldrich

Next Generation Cell Free Protein Expression Kit (Wheat Germ)

An Eukaryotic Cell Free Protein Expression system (CFPE/CFPS) with High Yield and Excellent Scalability, 50 reactions

別名:

cell free protein synthesis

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5 G
₩142,709
25 G
₩514,402
100 G
₩1,748,845
500 G
₩3,045,609

₩142,709


出荷予定日January 31, 2025詳細



サイズを選択してください

表示を変更する
5 G
₩142,709
25 G
₩514,402
100 G
₩1,748,845
500 G
₩3,045,609

About This Item

UNSPSCコード:
41105500
NACRES:
NA.56

₩142,709


出荷予定日January 31, 2025詳細


テクニック

protein expression: suitable

輸送温度

dry ice

保管温度

−70°C

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1 of 4

当該品目
D60015242331404
grade

for molecular biology

grade

-

grade

-

grade

-

technique(s)

FISH: suitable, FTIR: suitable

technique(s)

-

technique(s)

-

technique(s)

-

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL, clear, colorless to faintly greenish-yellow

solubility

H2O: 0.1 g/mL, clear, colorless to slightly yellow, water: 100 g/L at 25 °C (77 °F ), H2O: freely soluble 50 mg/mL, clear

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

200

form

powder

form

powder

form

powder

form

powder

詳細

Cell-free protein expression (CFPE) is the production of recombinant proteins in solution using biomolecular translation machinery extracted from cells of different species, such as E. Coli, wheat germ, Leishmania tarentolae, rabbit reticulocyte, insect or human cells. CFPE system from wheat germ extract is suitable for the expression of both prokaryotic and eukaryotic proteins.It usually does not require codon optimization even for the expression of transcripts with a high A/T or G/C content. CFPE system (wheat germ) provides best yield among the eukaryotic systems mentioned above. This system is particularly suitable for production of proteins with complicated conformation and multiple protein complexes, such as disulfide-bond-containing or integral membrane proteins. A variety of proteins and large proteins have been successfully produced from CFPE system (wheat germ), such as human or plant proteins or proteins for vaccine studies. For example, due to its high protein yield and easy to use, CFPE system (wheat germ) was selected as the most suitable choice for a comprehensive protein expression study in the ‘‘Human Protein Factory’’ project at the National Institute of Advanced Industrial Science and Technology (AIST) in Japan. In this study, the researchers tried to express 13364 human proteins, out of which 12996 clones (97.2%) produced protein in CFPE system (wheat germ). They also tested biological activities in two categories of the expressed proteins. Out of the 75 tested phosphatases, 58 (77%) showed biological activity. Also, several cytokines containing disulfide bonds that were expressed in a nonreducing CFPE system (wheat germ) were found in an active form.

Features and Benefits
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (part #: CFPS700) a next generation product protocol to ensure even higher stability, yield and more flexibility for a larger variety of target proteins, such as kinases, membrane proteins transcription factors, antibodies, photoreceptors etc., Our wheat germ extract contains various machines, micelle and organelles. As a result, even membrane protein can be soluble without adding any liposome and disulfide bonds are induced. By using this kit, a protein of interest can be expressed in a day starting from cDNA. There is no need for cloning or cell culture.

  • Great Time and cost efficiency, easy to use (batch model)
  • Excellent yield: 5.5mL sample scale per kit brings 0.5 mg to 1mg proteins to you in a day at low cost
  • No cloning or subcloning necessary for full length or truncation
  • Animal- and serum-free
  • Of eukaryotic origin
  • High solubility due to native conformation and eukaryotic source
  • Greater stability
  • Suitable for protein labeling and the expression of difficult-to-express proteins like membrane proteins and multiple protein complexes

Components
This product is comprised of the following:
  • 1x 25 mM NTP Mix
  • 1x T7 RNA Polymerase
  • 10x Transcription Buffer
  • 1x 100 mM DTT
  • 1x Wheat Germ Extract
  • 1x Amino Acid Mix

アプリケーション

  • Expression screening
  • High throughput in vitro production of recombinant proteins
  • Ribosome Display
  • Protein microarrays
  • Structural Genomics, NMR (Isotope labeling) or incorporating unnatural amino acid and crystallography

See Instruction for the Primer Design Tool for the 1st PCR
See CFPS700 Primer List (left-click and download the Excel file)

Storage and Stability

Store the components at the designated Temperatures once received.

10x Transcription Buffer: -20 °C
25 mM NTP Mix: -20 °C
T7 RNA Polymerase: -20 °C
100 mM DTT: -20 °C
Wheat Germ Extract: -70 °C
Amino Acid Mix: -70 °C

ピクトグラム

Corrosion

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Eye Dam. 1 - Skin Irrit. 2

保管分類コード

10 - Combustible liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

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PRTR

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消防法

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カルタヘナ法

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Jan Code

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資料

Cell-free protein synthesis rapidly produces recombinant proteins without cells, utilizing translation machinery from different cell types for efficiency.

プロトコル

Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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