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Key Documents

F7524

Sigma-Aldrich

Fetal Bovine Serum

non-USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Calf sera, Fetal Calf serum, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71

biological source

bovine fetus

Quality Level

400

sterility

sterile-filtered

product line

FBS Classic

composition

hemoglobin, ≤25 mg/dL

origin

non-USA origin

availability

not available in Canada, USA

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Fetal Bovine Serum has been used as a supplement in cell culture media such as Roswell Park Memorial Institute medium (RPMI-1640) and Dulbecco′s Modified Eagles Media (DMEM)to support the growth of a variety of cell lines.

Biochem/physiol Actions

Fetal bovine serum (FBS) serves as a commonly utilized cell culture media supplement to support in vitro and ex vivo cell, tissue, and organ cultures. FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth, and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth-promoting and survival enhancing factors to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. FBS is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Features and Benefits

F7524 is the same great product as Biochrom GmbH. product S0115.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Elisabeth F P Peterse et al.
BMC cancer, 16, 475-475 (2016-07-16)
Chondrosarcoma is a malignant cartilage forming bone tumour for which no effective systemic treatment is available. Previous studies illustrate the need for a better understanding of the role of the IGF pathway in chondrosarcoma to determine if it can be
Hans F N Kvitvang et al.
Journal of chromatography. A, 1370, 70-79 (2014-12-03)
Metabolic profiling has become an important tool in biological research, and the chromatographic separation of metabolites coupled with mass spectrometric detection is the most frequently used approach for such studies. The establishment of robust chromatographic methods for comprehensive coverage of
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Maria Peiris-Pagès et al.
Aging, 11(14), 4801-4835 (2019-07-18)
Using proteomics analysis, we previously compared MCF7 breast cancer cells grown as 3D tumor spheres, with the same cell line grown as monolayers. Our results indicated that during 3D anchorage-independent growth, the cellular machinery associated with i) mitochondrial biogenesis and
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Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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