MAB1592

Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1

ascites fluid, clone NP1, Chemicon^®

• 別名: Anti-CMT2CC, Anti-NFH
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: NP1, monoclonal
• application: ELISA; FACS; ICC; IHC; RIA; WB
• 化学種の反応性: bovine, rat, human, chicken
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); radioimmunoassay: suitable; western blot: suitable
• citations: 11

特性

• 由来生物: mouse
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: NP1, monoclonal
• 化学種の反応性: bovine, rat, human, chicken
• メーカー／製品名: Chemicon^®
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); radioimmunoassay: suitable; western blot: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_021076.2
• UniProtアクセッション番号: P07197; P12036
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: phosphorylation (not specified)
• 遺伝子情報: bovine ... Nefm(281347) ; chicken ... Nefm(396206) ; human ... NEFM(4741) ; rat ... Nefm(24588)

詳細

詳細

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

特異性

Recognizes the KSP repeats of the phosphorylated forms of neurofilament-M (NF-M) and neurofilament-H (NF-H), because NF-L does contain a few of these Lysine-serine-PO4-proline repeats some cross reactivity to NF-L may be seen at very high antibody concentrations.

免疫原

Bovine neurofilaments

Epitope: phosphorylated

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
ニューロフィラメント及び神経細胞代謝

神経細胞及びグリアマーカー

Detect Neurofilament H & M using this Anti-Neurofilament H & M Antibody, phosphorylated, clone NP1 validated for use in ELISA, FC, RIA, WB, IC, IH(P).

Western blot : recognizes, 200kDa, and 150kDa NF-H and NF-M bands primarily. Staining is removed with phosphatase treatment. Because NF-L also contains lysine-serine PO4 sites, NP1 may cross react with NF-L as well, but this has not be substantiated in traditional westerns.

Immunohistochemistry. Works on paraffin embedded, formalin fixed and frozen acteone fixed tissue sections. 1:50-1:500 for immunofluorescence.

Immunocytochemistry on PC12, TERA-2 and other neuronal lines.

FACS ELISA/RIA

Optimal working dilutions must be determined by end user.

関連事項

Replaces: 04-1061

物理的形状

Liquid. Contains no preservative.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable