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Merck

C7594

Sigma-Aldrich

カゼインブロッキングバッファー

for Northern and Southern blotting, powder blend

別名:

blocking buffer

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About This Item

EC Number:
UNSPSCコード:
12161700
NACRES:
NA.25

グレード

for molecular biology

品質水準

フォーム

powder blend

テクニック

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

溶解性

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

保管温度

room temp

詳細

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

アプリケーション

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

特徴および利点

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

調製ノート

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

保管分類コード

11 - Combustible Solids

WGK

WGK 1


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

C7594-BULK:
C7594-VAR:
C7594-1L:


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Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)
Serapion Pyrpassopoulos et al.
Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)
Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission
Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
W Querbes et al.
Journal of virology, 80(19), 9402-9413 (2006-09-16)
Invasion of glial cells by the human polyomavirus, JC virus (JCV), leads to a rapidly progressing and uniformly fatal demyelinating disease known as progressive multifocal leukoencephalopathy. The endocytic trafficking steps used by JCV to invade cells and initiate infection are
Marlène Cassar et al.
Frontiers in neuroscience, 14, 232-232 (2020-04-16)
A hallmark feature of Alzheimer's disease (AD) and other Tauopathies, like Frontotemporal Dementia with Parkinsonism linked to chromosome 17 (FTDP-17), is the accumulation of neurofibrillary tangles composed of the microtubule-associated protein Tau. As in AD, symptoms of FTDP-17 include cognitive

プロトコル

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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