おすすめの製品
詳細
Biotinylation is the process of labelling a molecule with a biotin molecule to take advantage of the strong binding and high specificity of biotin and streptavidin for purification or detection applications. Biotinylation is typically used to label proteins or nucleic acid based targets for these applications. Quantitation of biotinylation can be used to optimize the labelling of a given target. Levels of biotinylation can effect detection sensitivity or functionality of a purification product.
適合性
Suitable for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution
原理
This Colorimetric Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay utilizes HABA (4′-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm.
調製ノート
Storage and Stability: The kit is shipped on dry ice and storage at –20 °C, protected from light, is recommended.
Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Precautions and Disclaimer: This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
保管分類コード
10 - Combustible liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAK171-1KT:
この製品を見ている人はこちらもチェック
High-throughput biotinylation of proteins.
High Throughput Protein Expression and Purification, 185-198 (2009)
Site-specific biotinylation of purified proteins using BirA.
Fairhead M and Mark H
Site-Specific Protein Labeling, 171-184 (2015)
Imre Gaspar et al.
Bio-protocol, 8(5), e2750-e2750 (2018-03-05)
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using basic laboratory equipment to convert arrays of
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）