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B4639

Sigma-Aldrich

Biotin

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Synonym(s):
D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
Empirical Formula (Hill Notation):
C10H16N2O3S
CAS Number:
Molecular Weight:
244.31
Beilstein:
86838
EC Number:
MDL number:
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.21

product line

BioReagent

Quality Level

Assay

≥99%

form

powder

mol wt

Mw 244.31 g/mol

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

color

white to off-white

mp

231-233 °C (lit.)

solubility

H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)

storage temp.

2-8°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

InChI key

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

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This Item
B0301B4501B1116000
Biotin powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Sigma-Aldrich

B4639

Biotin

Biotin meets USP testing specifications

Sigma-Aldrich

B0301

Biotin

Biotin ≥99% (HPLC), lyophilized powder

Sigma-Aldrich

B4501

Biotin

Biotin European Pharmacopoeia (EP) Reference Standard

B1116000

Biotin

form

powder

form

powder

form

lyophilized powder

form

solid

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

-

technique(s)

HPLC: suitable

technique(s)

gas chromatography (GC): suitable, liquid chromatography (LC): suitable

color

white to off-white

color

white

color

white to off-white

color

-

mp

231-233 °C (lit.)

mp

231-233 °C (lit.)

mp

231-233 °C (lit.)

mp

231-233 °C (lit.)

solubility

H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)

solubility

-

solubility

ammonium hydroxide: 50 mg/mL (2 M)

solubility

-

General description

Biotin is also called as B complex vitamin. It is a bicyclic compound, which has a molecular weight of 244.31 Da. Biotin serves as an important cofactor for mammalian carboxylases. It is found within the cells in free and bound pools.

Application

Biotin has been used:
  • as a M199 media supplement in in vitro culture system
  • as a component of SATO serum free medium for the isolation of cortical oligodendrocyte precursors (OPCs)
  • in biochemical precursors culture for de novo fatty acid synthesis
  • as a component in aggregate culture media

Biochem/physiol Actions

Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
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25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Preparation of rat brain aggregate cultures for neuron and glia development studies
Koito H and Li J
Journal of Visualized Experiments, 31 (2009)
Convergent functional genomics of oligodendrocyte differentiation identifies multiple autoinhibitory signaling circuits
Gobert R P, et al.
Molecular and cellular biology, 29(6), 1538-1553 (2009)
Biochemical precursor effects on the fatty acid production in cell suspension cultures of Theobroma cacao L.
Parra O, et al.
Plant Physiology and Biochemistry, 111, 59-66 (2017)
Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
Mehta S R, et al.
Clinical and Vaccine Immunology : CVI, 15(12), 1764-1770 (2008)
Encyclopedia of dietary supplements (2010)

Articles

Biotin in Cell Culture

Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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