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V6629

Sigma-Aldrich

Vitamin B12

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%

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Synonym(s):
α-(5,6-Dimethylbenzimidazolyl)cyanocobamide, CN-Cbl, Cyanocob(III)alamin, Cyanocobalamin
Empirical Formula (Hill Notation):
C63H88CoN14O14P
CAS Number:
Molecular Weight:
1355.37
Beilstein:
4122889
EC Number:
eCl@ss:
34058004
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic ((organic))

Quality Level

product line

BioReagent

Assay

≥98%

form

powder

mol wt

Mw 1355.37 g/mol

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

color

red to brown

pH

6

solubility

water: soluble 50 mg/mL, dark red

storage temp.

2-8°C

SMILES string

C/C(C1=N[C@]([C@]([C@H](CC(N)=O)[C@@]/2(C)CCC(NC[C@@H](OP([O-])(O[C@H]3[C@@H](O)[C@@H](N4C=[N]5C6=C4C=C(C)C(C)=C6)O[C@@H]3CO)=O)C)=O)([H])N([Co+]5C#N)C2=C(C)/C([C@@H](CCC(N)=O)C/7(C)C)=NC7=C/8)(C)[C@@](C)(CC(N)=O)[C@@H]1CCC(N)=O)=C9N=C8[C@@H](CCC(N)=O)[C@

InChI

1S/C62H90N13O14P.CN.Co/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;1-2;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);;/q;;+2/p-2/t31-,34-,35-,36-,37+,41-,52-,53-,56-,57?,59-,60+,61+,62+;;/m1../s1

InChI key

RMRCNWBMXRMIRW-QJRSUKKJSA-L

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This Item
V2876C36071152011
Vitamin B12 BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%

Sigma-Aldrich

V6629

Vitamin B12

Vitamin B12 ≥98%

Sigma-Aldrich

V2876

Vitamin B12

Cyanocobalamin meets USP testing specifications

Sigma-Aldrich

C3607

Cyanocobalamin

Cyanocobalamin (crystallline) United States Pharmacopeia (USP) Reference Standard

USP

1152011

Cyanocobalamin (crystallline)

assay

≥98%

assay

≥98%

assay

96.0-102.0% dry basis

assay

-

form

powder

form

powder

form

powder

form

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

-

color

red to brown

color

red to brown

color

dark red

color

-

solubility

water: soluble 50 mg/mL, dark red

solubility

-

solubility

-

solubility

-

General description

Cyanocobalamin (B12) is a water soluble vitamin, commonly called as cobalamins. Dairy products, meat and eggs are the major sources of this vitamin.

Application

Vitamin B12 has been used:
  • as a component of Erdschreiber′s medium
  • as a supplement of Dulbecco′s modified Eagle′s medium used for the isolation of ventricular myocytes from neonatal and adult rats
  • orally administered to male quails to study its role in reducing the negative effects of sodium selenite intoxication, in vivo
  • as a supplement in DMEM/ medium 199 (M199) medium for differentiation of human myoblasts.

Biochem/physiol Actions

Vitamin B12 is metabolized to its active form, methylcobalamin. Hindered vitamin B12 uptake is observed in pernicious anemia. Vitamin B12 is essential for erythroblast development. Pregnancy reduces the levels of vitamin B12. Aging reduces vitamin B12 absorption. It works as a coenzyme in fat, carbohydrate and protein metabolism. It is required for growth, genetic stability and survival of cells in vitro. Functions to support one-carbon metabolism. Present in many classical and serum-free formulations.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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SAFC

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Vitamin B12 (cyanocobalamin)

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Articles

Vitamin B12, Cobalamin, in Cell Culture

Importance and uses of Vitamin B12 in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures

Folic Acid and Tetrahydrofolates in Cell Culture

Importance and uses of folic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures. Folic acid and its reduced derivative(s), such as folinic acid, are an essential water soluble vitamins added to virtually all cell culture media.

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