F2456

Fluorescein (free acid)

Dye content 95%, Powder

• Empirical Formula (Hill Notation): C₂₀H₁₂O₅
• CAS Number: 2321-07-5
• Molecular Weight: 332.31
• Colour Index Number: 45350:1
• Beilstein: 94324
• EC Number: 219-031-8
• MDL number: MFCD00005050
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894822
• NACRES: NA.47

Properties

• product name: Fluorescein (free acid), Dye content 95 %
• form: powder
• Quality Level: 200
• composition: Dye content, 95%
• technique(s): titration: suitable
• color: orange to red-brown
• mp: 320 °C (lit.)
• λmax: 496 nm
• ε (extinction coefficient): ≥12000 at 282-286 nm; ≥39000 at 236-240 nm; ≥70000 at 488-492 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
• InChI key: GNBHRKFJIUUOQI-UHFFFAOYSA-N

Description

General description

Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.

Application

Fluorescein (free acid) has been used as a staining agent in retinal imaging.

Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves