ECM550
QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
The CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Synonym(s):
Basement membrane cell invasion assay
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.84
Recommended Products
Quality Level
species reactivity
vertebrates
manufacturer/tradename
Chemicon®
QCM
technique(s)
cell based assay: suitable
detection method
colorimetric
shipped in
wet ice
General description
Also available: Cell Comb Scratch Assay! Get biochemical data from a scratch assay!Click Here
Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit′s performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.
Application:
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Introduction
Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.
The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit′s performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.
Application:
The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.
The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Application
Detection of migrating cells is via crystal violet stain.
Research Category
Cell Structure
Cell Structure
Packaging
12 wells
Components
Invasion Chamber: (Part No. 70019) One 24-well plate with 12 ECMatrixTM layered cell culture inserts.
Cell Stain: (Part No. 20294) One 10 mL bottle.
Cotton Swabs: (Part No. 10202) 24 each.
Forceps: (Part No. 10203) One each.
Cell Stain: (Part No. 20294) One 10 mL bottle.
Cotton Swabs: (Part No. 10202) 24 each.
Forceps: (Part No. 10203) One each.
Storage and Stability
Store kit materials at 2-8°C for up to 6 months. Do not freeze.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10-13 - German Storage Class 10 to 13
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Mullerian Inhibiting Substance inhibits invasion and migration of epithelial cancer cell lines.
Chang HL, Pieretti-Vanmarcke R, Nicolaou F, Li X, Wei X, Maclaughlin DT, Donahoe PK
Gynecologic Oncology null
Shanwu Ma et al.
Thoracic cancer, 11(3), 526-536 (2020-01-22)
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-associated mortality worldwide of which lung adenocarcinoma (LUAD) is the most common. The identification of oncogenes and effective drug targets is the key to individualized LUAD treatment. Actin-like protein 8
Inhibitory effect of norcantharidin, a derivative compound from blister beetles, on tumor invasion and metastasis in CT26 colorectal adenocarcinoma cells.
Yu-Jen Chen, Chwen-Jen Shieh, Tung-Hu Tsai, Cheng-Deng Kuo, Low-Tone Ho, Tsung-Yun Liu, Hui-Fen Liao
Anti-Cancer Drugs null
Carboxylated chitooligosaccharides (CCOS) inhibit MMP-9 expression in human fibrosarcoma cells via down-regulation of AP-1.
Niranjan Rajapakse, Moon-Moo Kim, Eresha Mendis, Ronghua Huang, Se-Kwon Kim
Biochimica et Biophysica Acta null
The inhibitory mechanism of a novel cationic glucosamine derivative against MMP-2 and MMP-9 expressions.
Eresha Mendis, Moon-Moo Kim, Niranjan Rajapakse, Se-Kwon Kim
Bioorganic & medicinal chemistry letters null
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