MABF3161

Anti-β-Catenin Antibody, clone 12F7

• Synonym(s): b-Cat
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 12F7, monoclonal
• mol wt: calculated mol wt 85.44 kDa; observed mol wt ~95 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): zebrafish, bovine, Xenopus, chicken, rat, mouse
• packaging: antibody small pack of 100 μg
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_990412
• UniProt accession no.: O42486
• shipped in: 2-8°C
• target post-translational modification: unmodified
• Gene Information: chicken ... B-Cat(395964)

Description

General description

β-Catenin (UniProt: O42486; also known as b-Cat) is encoded by the BCat gene (Gene ID: 395964) in chicken. Wnt/β-catenin signaling, a highly conserved pathway through evolution, regulates key cellular functions including proliferation, differentiation, migration, genetic stability, apoptosis, and stem cell renewal. β-catenin is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3β that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/β-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC β-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3. Mutations in β-catenin gene in humans are reported to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824; Pai, SC., et al. (2017). J. Hematol. Oncol. 10; Article 101; Johnson, KR., et al. (1993). Exp. Cell Res. 207(2); 252-260).

Specificity

Clone 12F7 is a mouse monoclonal antibody that detects β-catenin. It targets an epitope within 22 amino acids from the N-terminal half.

Immunogen

MBP-conjugated recombinant full-length chicken β-catenin.

Application

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysates.

Western Blotting Analysis: A 1:125 dilution of this antibody detected β-Catenin in A431 cell lysates.

Tested Applications

Immunohistochemistry Applications: A representative lot detected β-Catenin in Immunohistochemistry applications (Qu, Y., et al. (2016). Proc. Natl. Acad. Sci. USA.113(33); 9339-44).

Western Blotting Analysis: A representative lot detected β-Catenin in Western Blotting applications (Johnson, K.R., et al. (1993). Exp. Cell Res. 207(2); 252-60).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-β-Catenin, clone 12F7, Cat. No. MABF3161, is a mouse monoclonal antibody that detects -β-Catenin and is tested for use in Immunohistochemistry and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable