MABT134
Anti-VE-cadherin Antibody, clone BV6
clone BV6, from mouse
Synonym(s):
Cadherin-5, 7B4 Antigen
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BV6, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CDH5(1003)
General description
Human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell-to-cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. VE-cadherin also associates with alpha-catenin forming a link to the cytoskeleton.
Immunogen
HUVEC cells
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected VE-cadherin in the cell-cell junctions of HUVEC cells.
Immunohistochemistry Analysis: A 1:10-1:20 dilution of a representative lot was used by an independent laboratory in paraffin-embedded tissue sections. Buffered formalin fixation is recommended with a fixation period of no longer than 12 hours. High heat antigen retrieval in citrate buffer (Cat. No. 21545) is also suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells using a immunoperoxidase staining protocol (eg. IHC Select Detection Kit, Cat. No. DAB150)
Flow Cytometry Analysis: A representative lot was used in the presence of Ca2+. Note: Use PBS + 2-5mM EDTA for cell detachment.
Western Blot Analysis: Non-reducing conditions may be needed, Ca2+ required in buffer (2-5 mM).
Immunohistochemistry Analysis: A 1:10-1:20 dilution of a representative lot was used by an independent laboratory in paraffin-embedded tissue sections. Buffered formalin fixation is recommended with a fixation period of no longer than 12 hours. High heat antigen retrieval in citrate buffer (Cat. No. 21545) is also suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells using a immunoperoxidase staining protocol (eg. IHC Select Detection Kit, Cat. No. DAB150)
Flow Cytometry Analysis: A representative lot was used in the presence of Ca2+. Note: Use PBS + 2-5mM EDTA for cell detachment.
Western Blot Analysis: Non-reducing conditions may be needed, Ca2+ required in buffer (2-5 mM).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-VE-cadherin Antibody, clone BV6 is validated for use in WB, IC, FC, IH(P) for the detection of VE-cadherin.
Quality
Evaluated by Western Blot in HUVEC cell lysate.
Western Blot Anlaysis: A 1:2,000 dilution of this antibody detected VE-cadherin in HUVEC cell lysate.
Western Blot Anlaysis: A 1:2,000 dilution of this antibody detected VE-cadherin in HUVEC cell lysate.
Target description
~120 kDa observed.
The calculated molecular weight is 82 kDa but it will run between ~90-140 kDa because this protein is glycosolated.
The calculated molecular weight is 82 kDa but it will run between ~90-140 kDa because this protein is glycosolated.
Linkage
Replaces: MAB1989
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HUVEC cell lysate
HUVEC cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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