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PIHP01250

Millipore

Millicell® Standing Cell Culture Inserts

pore size 0.4 μm, diam. 12 mm, translucent polycarbonate membrane, Tissue Culture (TC)-treated surface, size 24 wells, sterile

Synonym(s):

Millicell Cell Culture Insert, 12 mm, polycarbonate, 0.4 µm, Millicell-PCF, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

polystyrene housing
translucent polycarbonate membrane

Quality Level

sterility

γ-irradiated
sterile

feature

hydrophilic
100x106 pores/cm2 (pore density)

packaging

pack of 50

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

H

10.5 mm

diam.

12 mm

size

24 wells

surface area

0.6 cm2

working volume

0.6 mL

color

translucent

matrix

Isopore

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

General description

Device Configuration: 24-well plate, 0.4μm pore size

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Millicell® Standing Inserts:
- Promotes excellent cell growth and provides an exceptional opportunity for cell studies

Membrane Type:
Isopore Membrane (polycarbonate)
- For growth of attachment-dependent cells without matrix

Applications
Cell Attachment, Cell Growth, Cell Differentiation , Immunocytochemistry

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Susanne Elisabeth Pors et al.
Molecular human reproduction, 26(5), 301-311 (2020-03-24)
In vitro activation of resting ovarian follicles, with the use of mechanical stress and/or pharmacological compounds, is an emerging and novel approach for infertility treatment. The aim of this study was to assess the sphingolipid, sphingosine-1-phosphate (S1P), as a potential
Peiying Tian et al.
Cell biology international, 44(8), 1681-1690 (2020-04-14)
Ghrelin-O-acyltransferase (GOAT) is a membrane-bound enzyme that attaches eight-carbon octanoate to a serine residue in ghrelin and thereby acylates inactive ghrelin to produce active ghrelin. In this study, we investigated the function of GOAT in the intestinal mucosal barrier. The

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16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

Protocols

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

This protocol covers 3 modes for the microscopic examination of cell samples.

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

See All

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