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S-002-M

Sigma-Aldrich

Cell Culture Freezing Media

contains DMSO, liquid

Synonym(s):

Cell Culture Freezing Media - DMSO, liquid, cell cryopreservation medium

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.24

Quality Level

200

form

liquid

manufacturer/tradename

Specialty Media

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

input

sample type cardiac stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)

Related Categories

General description

This unique formulation of cell culture freezing media is for cryopreservation of a broad spectrum of mammalian cells (see viability data). The reagents used in these products are certified to be virus and mycoplasma free.



Contains 10% DMSO, calf & fetal bovine serum

Application

Cell Culture Freezing Media - DMSO, liquid has been used:
  • to dissolve temozolomide (TMZ), a DNA methylating agent, for treating U251 cells
  • in 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay to assess cell viability of TMZ treated U251 cells
  • for reconstituting RepSox (a Transforming growth factor (TGF)-β/ALK5 inhibitor) and SB431542 (TGF-β inhibitor) to prepare stock solutions
  • as vehicle control for the measurement of transendothelial electrical resistance (TEER) time-course in human pluripotent stem cell-derived endothelial cells (hPSC-ECs) treated with RepSox and SB431542

Features and Benefits

The cell culture Freezing Media-DMSO, liquid is available in a 50 mL format & has been optimized & validated for cell culture.

Physical form

Liquid with Contains 10% DMSO, calf and fetal bovine serum

Storage and Stability

Store at -20°C upto 2 years.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lei Wang et al.
Biochemical and biophysical research communications, 504(1), 46-53 (2018-09-03)
To investigate the effects and underlying molecular mechanisms of FoxG1 expression on glioblastoma multiforme (GBM) models. Expression levels of FoxG1 and other cancer-related biomarkers were evaluated by qRT-PCR, immunoblotting and immunohistochemistry. Crystal violet staining and MTT assay and were applied
Bo Kyoung Kim et al.
Methods in molecular biology (Clifton, N.J.), 2475, 259-274 (2022-04-23)
Difficulties with poor reproducibility and translatability of animal model-based research, along with increased efforts to abide by the 3Rs tenet of animal welfare, are driving demand for more relevant human cellular systems. This is especially true for central nervous system

Articles

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

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