S4030

Dextran Sulfate Solution

50% (w/v) liquid

• Synonym(s): dextran sulfate buffer, hybridization accelerant, hybridization additive, northern blot hybridization component, southern blot hybridization component
• UNSPSC Code: 41116012
• eCl@ss: 32160405
• NACRES: NA.52

Properties

• product name: Dextran Sulfate 50% Solution,
• packaging: pkg of 100 mL
• Quality Level: 100
• manufacturer/tradename: Chemicon^®
• technique(s): hybridization: suitable (nucleic acid)
• shipped in: ambient

Description

General description

Dextran Sulfate (MW >500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) solution.

Application

Dextran Sulfate - 50% Solution

To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.



See the "Hybrisol Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4030 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2