UFC200324

Amicon^® Ultra Centrifugal Filter, 3 kDa MWCO

sample volume 2 mL, regenerated cellulose membrane, MWCO 3 kDa

• Synonym(s): Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume
• UNSPSC Code: 41104916
• eCl@ss: 36100101
• NACRES: NB.24

Properties

• material: polypropylene tube (for collection); regenerated cellulose membrane; styrene-butadiene housing
• Quality Level: 400
• sterility: non-sterile
• product line: Amicon^®
• manufacturer/tradename: Amicon^® Ultra
• parameter: 2 mL sample volume
• technique(s): protein extraction: suitable; protein purification: suitable
• L: 119.7 mm
• diam.: 15.9 mm
• filtration area: 1 cm²
• volume: 2 mL
• pore size: 3 kDa MWCO
• shipped in: ambient

Description

General description

Amicon^® Ultra-2 mL centrifugal devices with Ultracel^® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at 100% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon^® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^® Ultra 2 mL filters deliver the highest recovery with shortest spin times.

Application

• Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel^® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates.
• Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

Features and Benefits

• Ultracel^® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%
• Concentrates 2 mL down to 15-70 μL
• 98% sample recovery
• Fast processing time: 10 to 30-minute spin times
• Engineered dead stop
• Reverse spin capability provides consistent recoveries

Linkage

Replaces: 4202

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany