EHIFI-RO

Expand^™ High Fidelity PCR System

sufficient for ≤40 reactions (11732641001), sufficient for ≤200 reactions (11732650001), sufficient for ≤1,000 reactions (11759078001)

• Synonym(s): high fidelity pcr system, expand
• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC Code: 41106300

Properties

• usage: sufficient for ≤1,000 reactions (11759078001); sufficient for ≤200 reactions (11732650001); sufficient for ≤40 reactions (11732641001)
• Quality Level: 100
• packaging: pkg of 2,500 U (11759078001 [10x250 U]); pkg of 100 U (11732641001); pkg of 500 U (11732650001 [2x250 U])
• manufacturer/tradename: Roche
• technique(s): PCR: suitable
• storage temp.: −20°C

Description

General description

Expand High Fidelity PCR System is optimized to efficiently amplify DNA fragments up to 5 kb. PCR up to 9 kb is possible, with yield diminishing as DNA fragment length increases. The Expand High Fidelity PCR System is composed of an enzyme mix containing thermostable Taq DNA polymerase and a thermostable DNA polymerase with proofreading activity. The fidelity of DNA synthesis with Expand High Fidelity PCR System shows a threefold increase compared to Taq DNA polymerase. T/A cloning is recommended.

The 5′ → 3′ polymerase activity of Taq DNA Polymerase and the 3′ → 5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′ → 5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.

Application

The Expand^™ High Fidelity PCR System is an enzyme blend delivering superior results with twofold higher yield and threefold greater fidelity compared to Taq DNA Polymerase. Choose Expand High Fidelity PCR System for the most sensitive PCR results for genomic targets up to 5 kb in length in:

• PCR
• RT-PCR

Use Expand High Fidelity PCR System, dNTPack with ready-to-use PCR nucleotide mix.
For maximum convenience, select the 2x concentrated ready-to-use High Fidelity Master.

Features and Benefits

Expand High Fidelity PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity for high yield and fidelity. It is optimized for PCR of DNA fragments up to 5 kb.
Use 2.6 U for a standard 50 μl PCR.

Volume activity: 3.5 U/μl

• Use one enzyme for all applications.

Enjoy high yield, fidelity, and flexibility.

• Detect PCR products

previously undetectable and avoid false negatives.

• Achieve successful results

from small quantities of template DNA.

Packaging

1 kit containing 4 components

Quality

Each lot of Expand High Fidelity is function-tested in PCR using human genomic DNA and specific primers for collagen (1 kb amplified product) and the tPA gene (4.8 kb amplified product).

Unit Definition

Volume Activity: 3.5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Expand is a trademark of Roche

Kit Components Only

• Expand High Fidelity Enzyme mix
• Expand High Fidelity Buffer, with 15 mM MgCl₂ 10x concentrated
• Expand High Fidelity Buffer, without MgCl₂ 10x concentrated
• MgCl₂ Stock Solution 25 mM

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash