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63052

Sigma-Aldrich

Magnesium acetate solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):

Magnesium acetate anhydrous, Mg(OAc)2

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About This Item

Linear Formula:
(CH3COO)2Mg
CAS Number:
142-72-3
Molecular Weight:
142.39
Beilstein:
3692530
MDL number:
MFCD00003539
UNSPSC Code:
12161700
PubChem Substance ID:
329759766
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

form

liquid

concentration

~1 M in H2O

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.358

pH

5.0-7.0 (25 °C)

cation traces

Al: ≤5 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤5 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mn: ≤5 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤2 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.02

SMILES string

CC(=O)O[Mg]OC(C)=O

InChI

1S/2C2H4O2.Mg/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+2/p-2

InChI key

UEGPKNKPLBYCNK-UHFFFAOYSA-L

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Application

Magnesium acetate is used in molecular biology applications such as batch in vitro transcription of RNA and the crystallization of transcription factor:DNA complexes and proteins via the sitting-drop vapor-diffusion method.
Magnesium acetate solution has been used:
  • to supplement Midkin fused to the biotin ligase BioID2 (MK-BioID2) conditioned medium for in-tube biotinylation assay
  • as a component of lysis buffer and amplified RNA (aRNA) fragmentation buffer to perform aRNA fragmentation
  • as a component of lysis buffer and DamID buffer for sequencing zygote samples

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL3788
BCCJ9123
BCCH2942
BCCH5826
BCCG7154

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Roberto Stella et al.
Analytical and bioanalytical chemistry, 408(29), 8343-8353 (2016-10-04)
The illegal use of pharmacologically active compounds for growth promotion in food-producing species poses risks for consumer health and animal welfare. Surveillance relies on the quantification of drug residues in animal fluids or tissues, but the efficacy can be negatively
Navneet Bung et al.
Scientific reports, 8(1), 9599-9599 (2018-06-27)
AMPK is considered as a potential high value target for metabolic disorders. Here, we present the molecular modeling, in vitro and in vivo characterization of Activator-3, 2-[2-(4-(trifluoromethyl)phenylamino)thiazol-4-yl]acetic acid, an AMP mimetic and a potent pan-AMPK activator. Activator-3 and AMP likely
Linda E Franken et al.
Nature communications, 8(1), 722-722 (2017-09-30)
Bacteria downregulate their ribosomal activity through dimerization of 70S ribosomes, yielding inactive 100S complexes. In Escherichia coli, dimerization is mediated by the hibernation promotion factor (HPF) and ribosome modulation factor. Here we report the cryo-electron microscopy study on 100S ribosomes
Cinthia R Millan et al.
Nucleic acids research, 45(14), 8378-8391 (2017-06-24)
Trypanosoma brucei, the causative agent of sleeping sickness (Human African Trypanosomiasis, HAT), contains a kinetoplast with the mitochondrial DNA (kDNA), comprising of >70% AT base pairs. This has prompted studies of drugs interacting with AT-rich DNA, such as the N-phenylbenzamide
Quentin M Dudley et al.
ACS synthetic biology, 5(12), 1578-1588 (2016-08-02)
Cell-free metabolic engineering (CFME) is advancing a powerful paradigm for accelerating the design and synthesis of biosynthetic pathways. However, as most cell-free biomolecule synthesis systems to date use purified enzymes, energy and cofactor balance can be limiting. To address this
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