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AEC101

Sigma-Aldrich

AEC Staining Kit

liquid

Synonym(s):

ACE substrate kit

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About This Item

UNSPSC Code:
41116134
NACRES:
NA.32

form

liquid

Quality Level

200

usage

sufficient for ≥1,000 tests (150 ml substrate solution)

storage temp.

2-8°C

Application

AEC Staining Kit has been used as a substrate
  • for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods
  • for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay
  • for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads

Packaging

Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.

Caution

Stable at least one year at 2-8 °C.

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Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

134.6 °F - closed cup

Flash Point(C)

57 °C - closed cup

Certificates of Analysis (COA)

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Kuangda Lu et al.
Journal of the American Chemical Society, 138(38), 12502-12510 (2016-08-31)
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Leslie R Morse et al.
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Uncoupling of inflammation and insulin resistance by NF-kappaB in transgenic mice through elevated energy expenditure
Tang T, et al.
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Identification of early secretory antigen target-6 epitopes for the immunodiagnosis of active tuberculosis
Vincenti D, et al.
Molecular Medicine, 9(3), 105-105 (2003)
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Checkpoint blockade immunotherapy (CBI) elicits durable therapeutic responses by blocking T cell inhibitory pathways of tumors with pre-infiltrated T cells and/or high mutational burden to activate antitumor immunity but is ineffective against poorly immunogenic tumors. Immunogenic radiotherapy, photodynamic therapy (PDT)
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Protocols

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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