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B1125

Sigma-Aldrich

Bathocuproinedisulfonic acid disodium salt

7.2-9.1% purity dry basis (ICP), powder

Synonym(s):

2,9-Dimethyl-4,7-diphenyl-1,10-phenantrolinedisulfonic acid disodium salt, Disodium bathocuproinedisulfonate

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About This Item

Empirical Formula (Hill Notation):
C26H18N2Na2O6S2
CAS Number:
Molecular Weight:
564.54
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Bathocuproinedisulfonic acid disodium salt, for spectrophotometric det. of Cu, Fe

Assay

7.2-9.1% dry basis (ICP)

Quality Level

form

powder

quality

for spectrophotometric det. of Cu, Fe

technique(s)

titration: 90% using HCLO4 (Dry Basis)

color

white to beige

solubility

H2O: 50 mg/mL

suitability

suitable for determination of copper

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H20N2O6S2.2Na/c1-15-25(35(29,30)31)21(17-9-5-3-6-10-17)19-13-14-20-22(18-11-7-4-8-12-18)26(36(32,33)34)16(2)28-24(20)23(19)27-15;;/h3-14H,1-2H3,(H,29,30,31)(H,32,33,34);;/q;2*+1/p-2

InChI key

RNGKZLRAVYPLJC-UHFFFAOYSA-L

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General description

Bathocuproinedisulfonic acid is a strong copper chelator. It is used for the determination of copper and iron in biological samples.

Application

  • Bathocuproinedisulfonic acid disodium salt has been used as a copper chelator for specific assay in Arabidopsis seedlings.
  • It has been used as an electron chain-associated free radical production inhibitor for superoxide dismutase assay in cells.
  • It has been added to the culture media of trypanosomes as a copper chelator for the stabilization of cysteine.

Other Notes

Mixture of isomers

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shinya Ogawa et al.
Analytical chemistry, 81(21), 9199-9200 (2009-09-30)
Reoptimization of analytical conditions was performed for a high-performance liquid chromatographic (HPLC) detection system for Cu(I) chelators based on the dequenching of Cu(I)-bathocuproine disulfonate complexes that occurs in the presence of Cu(I) chelators. The revision corrects for emission and excitation
Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue.
Weydert CJ and Cullen JJ
Nature Protocols, 5, 51-66 (2010)
Shinya Ogawa et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 909, 34-36 (2012-11-17)
HPLC eluent systems employing acetonitrile and methanol were evaluated for the quantitation of glutathione (GSH) and phytochelatin (PC(n)), a family of peptides implicated in heavy-metal detoxification in higher plants. The detection system is based on the dequenching of copper(I)-bathocuproine disulfonate
Tomoo Shirabe et al.
Analytical chemistry, 80(23), 9360-9362 (2009-06-25)
A novel method has been devised for the determination of phytochelatins (PCs), heavy-metal-tolerant peptides produced by higher plants and algae. The method is based on the facts that fluorescence of bathocuproine disulfonate (BCS) is quenched by Cu(I) ions as a
Simple procedure for the single sample determination of serum copper and iron.
B ZAK
Clinica chimica acta; international journal of clinical chemistry, 3(4), 328-334 (1958-07-01)

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