B2518

Albumin, Acetylated from bovine serum

lyophilized powder, suitable for microbiology

• Synonym(s): Ac-BSA, Acetyl BSA, Acetylated BSA
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD01114300
• UNSPSC Code: 12352202
• NACRES: NA.31

Properties

• biological source: bovine
• Quality Level: 200
• form: lyophilized powder
• purified by: heat shock fractionation
• origin: USA origin
• technique(s): Southern blotting: suitable; blocking: suitable; cell culture | mammalian: suitable
• impurities: HIV I and HIVII, HCV and HBsAg, tested negative
• pH: 7
• suitability: suitable for microbiology
• UniProt accession no.: P02769
• foreign activity: DNase free ; DNase, RNase, NICKase, protease, none detected; NICKase free ; Protease free ; RNase free
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

General description

Native bovine serum albumin (BSA) is a 66 kDa protein, which belongs to the family of serum albumin. It consists of three domains (I, II and III) and is the major protein found in circulatory system. Native BSA is an α-helical, globular and non-glycosylated protein, with 17-disulfide bonds.

Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.

Application

Select the right albumin for your application with the Albumin Selection Guide.

Acetylated bovine serum albumin has been used:

• as a component of protease buffer for protease inhibition assay
• as a blocking agent in the preparation of cDNA glass array
• to select hybridoma antibodies sensitive to M-ficolin epitopes by immunofluorometric assay

This B2518 acetylated BSA product has been cited in various publications and protocols, such as the following:






* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)




* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)




* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)




* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)

Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Biochem/physiol Actions

Native bovine serum albumin (BSA) acts as a transporter of drugs, hormones and fatty acids. It has calcium binding sites and regulates metal homeostasis.

Features and Benefits

• Acetylated to inactivate trace nucleases
• DNase free / Exonuclease free
• Nickase free / Endonuclease free
• RNase free
• Protease free

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves