Skip to Content
Merck
All Photos(3)

Key Documents

D2197

Sigma-Aldrich

Diaphorase from Clostridium kluyveri

recombinant, expressed in E. coli, Animal-component free

Synonym(s):

Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase

Sign Into View Organizational & Contract Pricing

Select a Size

5 G
$233.00

$233.00


In Stock


Request a Bulk Order

Select a Size

Change View
5 G
$233.00

About This Item

Enzyme Commission number:
UNSPSC Code:
12352204
NACRES:
NA.54

$233.00


In Stock


Request a Bulk Order

recombinant

expressed in E. coli

Quality Level

form

solid

specific activity

>=30 units/mg protein (biuret)

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A4503A3733A2934
Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

General description

Research area: Cancer

Application

Diaphorase from Clostridium kluyveri has been used:
  • in lactate dehydrogenase assay in gill cells.[1]
  • impregnated on the strip for formate dehydrogenase assay.[2]
  • in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
  • for semiquinone generation during benzoquinone reduction.[3]
  • for evaluation of glucose uptake in cultured muscle cells.[4]
  • in lactate dehydrogenase cytotoxicity assay.[5]

Biochem/physiol Actions

Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.

Unit Definition

One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.

Physical form

Supplied as a lyophilized powder containing trehalose.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Apor Veres-Székely et al.
    International journal of molecular sciences, 23(4) (2022-02-27)
    Fibroblasts play a central role in diseases associated with excessive deposition of extracellular matrix (ECM), including idiopathic pulmonary fibrosis. Investigation of different properties of fibroblasts, such as migration, proliferation, and collagen-rich ECM production is unavoidable both in basic research and
    Relationship between insulin sensitivity and gene expression in human skeletal muscle
    Parikh HM, et al.
    BMC endocrine disorders, 21, 32-32 (2021)
    Claudio F Gonzalez et al.
    The Journal of biological chemistry, 280(24), 22590-22595 (2005-04-21)
    Most bacteria contain soluble quinone-reducing flavoenzymes. However, no biological benefit for this activity has previously been demonstrated. ChrR of Pseudomonas putida is one such enzyme that has also been characterized as a chromate reductase; yet we propose that it is
    Knut Erik Hovda et al.
    Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
    The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the
    Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration
    Veres-Szekely A, et al.
    International Journal of Molecular Sciences, 23(4), 2119-2119 (2022)

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service