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F6178

Sigma-Aldrich

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤25 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

≤25 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bon-Kyoung Koo et al.
Nature, 488(7413), 665-669 (2012-08-17)
LGR5+ stem cells reside at crypt bottoms, intermingled with Paneth cells that provide Wnt, Notch and epidermal growth factor signals. Here we find that the related RNF43 and ZNRF3 transmembrane E3 ubiquitin ligases are uniquely expressed in LGR5+ stem cells.
Li Ding et al.
Autophagy, 13(7), 1176-1190 (2017-06-09)
Although the treatments of malignant glioma include surgery, radiotherapy and chemotherapy by oral drug administration, the prognosis of patients with glioma remains very poor. We developed a polyethylene glycol-dipalmitoylphosphatidyle- thanoiamine (mPEG-DPPE) calcium phosphate nanoparticles (NPs) injectable thermoresponsive hydrogel (nanocomposite gel)
Martina Belli et al.
Endocrinology, 159(4), 1690-1703 (2018-02-23)
Germline knockout studies in female mice demonstrated an essential role for forkhead box L2 (FOXL2) in early follicle development, whereas an inducible granulosa cell (GC)-specific deletion of Foxl2 in adults has shown ovary-to-testis somatic sex reprogramming. In women, over 120
Y Zhou et al.
Oncogene, 36(6), 731-745 (2016-08-02)
microRNA (miRNA) dysregulation is a common feature of cancer cells, but the complex roles of miRNAs in cancer are not fully elucidated. Here, we used functional genomics to identify oncogenic miRNAs in non-small cell lung cancer and evaluate their impact
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Articles

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Protocols

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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