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Key Documents

L6533

Sigma-Aldrich

Lectin from Dolichos biflorus (horse gram)

biotin conjugate, buffered aqueous solution

Synonym(s):

Dolichos biflorus agglutinin, DBA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

biotin conjugate

Quality Level

200

form

buffered aqueous solution

composition

Protein, ≥2.5 mg/mL biuret

extent of labeling

≥3 mol biotin per mol lectin

storage temp.

−20°C

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General description

Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.

Application

Lectin from Dolichos biflorus (horse gram) has been used:
  • to characterize mouse uterine natural killer (uNK) cells
  • in double staining with lectins to localize nuclear factor-κB (NF-κB) activation
  • as a species-specific gonocyte marker for the determination of donor cell colonization
  • to label undifferentiated spermatogonia/cat testicular tissue

Biochem/physiol Actions

Dolichos biflorus agglutinin (DBA) possesses binding activity for which it is used as a marker binucleate cells and can indicate the state of their differentiation. It serves as a label for primordial germ cells (PGCs), gonocytes, pre-spermatogonia and spermatogonia.
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Packaging

Sold on the basis of mg active conjugate.

Physical form

contains 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca2+

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tubular NF-kB and AP-1 activation in human proteinuric renal disease
Mezzano SA, et al.
Kidney International, 60(4), 1366-1377 (2001)
Effect of horse gram lectin (Dolichos biflorus agglutinin) on degranulation of mast cells and basophils of atopic subjects: Identification as an allergen
Pramod SN, et al.
International Immunopharmacology, 6(11), 1714-1722 (2006)
Bovine sertoli cells colonize and form tubules in murine hosts following transplantation and grafting procedures
Zhang Z, et al.
Journal of Andrology, 29(4), 418-430 (2008)
Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells
Nakano H, et al.
Reproduction (Cambridge, England), 124(4), 581-592 (2002)
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
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