M7167

M2 medium

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

• Synonym(s): Cell Culture M2, Cell Culture Medium, M2 Medium Solution
• MDL number: MFCD00283761
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• form: liquid
• storage condition: protect from light
• technique(s): cell culture | embryo: suitable
• impurities: endotoxin, tested
• components: HEPES: 5.42726 g/L; NaHCO₃: 0.35 g/L; glucose: 1.0 g/L (Dextro); phenol red: 0.0106 g/L; sodium pyruvate: 0.0363 g/L
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Quality

Presence of particulates in solution will not affect product′s performance.

Reconstitution

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable