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Merck

MP0030

Sigma-Aldrich

LookOut® Mycoplasma Elimination Kit

Synonym(s):

Mycoplasma contamination treatment

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

usage

 kit sufficient for 5 mL mycoplasma elimination

Quality Level

packaging

pkg of 1 kit

storage condition

protect from light

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

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This Item
MP0035L8917TOSRTRO
usage

 kit sufficient for 5 mL mycoplasma elimination

usage

-

usage

-

usage

sufficient for 150 reactions, sufficient for 50 reactions

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

packaging

pkg of 150 reactions (04655885001), pkg of 50 reactions (04655877001)

storage condition

protect from light

storage condition

-

storage condition

dry at room temperature

storage condition

-

mycoplamsa removal agent, MRA,

mycoplasma detection, PCR-based assay

DNA decontamination, surface decontamination, DNA destroy

-

application(s)

detection
microbiology

application(s)

detection
microbiology

application(s)

microbiology

application(s)

-

General description

The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.

Application

The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.

Features and Benefits

  • Minimal cytotoxic properties of the reagents
  • Highly effective
  • Retention of normal cell characteristics

Suitability

The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.

Legal Information

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.

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Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids


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Find documentation for the products that you have recently purchased in the Document Library.

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Optimized PCR-based Detection of Mycoplasma
Dobrovolny PL and Bess D
Journal of Visualized Experiments, 52 (2011)

Articles

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Overview of good cell banking practices for cell line cryopreservation purposes.

See All

Protocols

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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