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N7660

Sigma-Aldrich

4-Nitrophenol

chromogenic, liquid

Synonym(s):

p-Nitrophenol solution

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About This Item

Empirical Formula (Hill Notation):
C6H5NO3
CAS Number:
100-02-7
Molecular Weight:
139.11
MDL number:
MFCD00007331
UNSPSC Code:
12352204
eCl@ss:
39032018
PubChem Substance ID:
57654462
NACRES:
NA.28

product name

4-Nitrophenol solution, 10 mM

form

liquid

concentration

10 mM

storage temp.

2-8°C

SMILES string

Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H5NO3/c8-6-3-1-5(2-4-6)7(9)10/h1-4,8H

InChI key

BTJIUGUIPKRLHP-UHFFFAOYSA-N

Gene Information

human ... UGT1A4(54657)

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General description

4-Nitrophenol is a phenolic compound present as a chemical intermediate and breakdown product during the production of organophosphorus insecticide derivatives of parathion. It is an intermediate during the synthesis of paracetamol. 4-Nitrophenol is a chromogenic catalytic by product of the synthetic substrate 4-nitrophenyl phosphate. It is majorly used as a reference standard in alkaline phosphatase assay. 4-Nitrophenol is nonbiodegradable and its derivatives are used for herbicide and fungicide production.

Application

4-Nitrophenol solution has been used:
  • in the generation of standard curve for quantifying alkaline phosphatase activity of mesenchymal stem cells
  • bone marrow stromal cells (BMSCs)
  • for quantifying β-hexosaminidase activity in HeLa cells
  • as a standard for calorimetric determination of alkaline phosphatase activity at 405 nm
  • for assessing the β-hexosamindase activity

Preparation Note

Prepared as a 1.39 mg/ml solution suitable for use as a calibrator in alkaline phosphatase assays (such as Bessey-Lowry-Brock method) using p-nitrophenol phosphate as a substrate.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phosphatase substrate powder

Sigma-Aldrich

P4744

Phosphatase substrate

Competitive flow immunoassay with fluorescence detection for determination of 4-nitrophenol.
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