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O7252

Sigma-Aldrich

Orange G

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G

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About This Item

Empirical Formula (Hill Notation):
C16H10N2Na2O7S2
CAS Number:
Molecular Weight:
452.37
Colour Index Number:
16230
Beilstein:
4120705
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Orange G, certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥80%

color

orange to very dark orange

solubility

water: 1 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

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General description

Orange G (C.l. 16230) is the disodium salt of benzeneazo-2-naphthol-6,8-disulfonic acid, C16H10N2O7S2Na2. Orange G, also known as wool orange 2G, is a component in several industry applications including cytology, histology, biology, and industrial staining. It is a component of Mallory′s connective tissue stain.

Application

Orange G is typically used as a background or cytoplasmic stain for various biological samples including a common application within clincial cytology within Papanicolaou varients.

Suitability

Certified for use in Mallory′s connective tissue stain; Wilson-Ezrin method for pituitary acidophils; Kreyberg′s method for keratin and mucus; as a counterstain following Heidenhain′s iron hematoxylin on paraffin sections; Flemming′s triple stain on paraffin sections of onion root.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen.
Givens RM
BMC Research Notes, 4, 499-499 (2011)
Asli Yuksel et al.
Journal of hazardous materials, 190(1-3), 1058-1062 (2011-03-29)
Complete degradation of azo dye Orange G was studied using a 500 mL continuous flow reactor made of SUS 316 stainless steel. In this system, a titanium reactor wall acted as a cathode and a titanium plate-type electrode was used
M Arulkumar et al.
Journal of hazardous materials, 186(1), 827-834 (2011-01-15)
Thespesia populnea is a large tree found in the tropical regions and coastal forests of India. Its pods were used as a raw material for the preparation of activated carbon. The prepared activated carbon was used for the adsorptive removal
Arumugam Sivasamy et al.
Current microbiology, 62(2), 351-357 (2010-07-21)
Biosorption is an eco-friendly and cost-effective method for treating the dye house effluents. Aspergillus niger and Trichoderma sp. were cultivated in bulk and biomasses used as biosorbents for the biosorption of an azo dye Orange G. Batch biosorption studies were
G Thennarasu et al.
Environmental science and pollution research international, 19(7), 2755-2765 (2012-02-23)
The photocatalytic degradation of Orange G (OG) dye has been investigated using synthesised nanocrystalline ZnO as a photocatalyst and sunlight as the irradiation source. The formation of ZnO prepared from its precursor was confirmed through FT-IR and powder X-ray diffraction

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