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Key Documents

P0029

Sigma-Aldrich

Protease from Bacillus sp.

Synonym(s):

Protamex®

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About This Item

Enzyme Commission number:
3.4.21.14
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Bacillus sp.

Quality Level

form

granular

enzyme activity

>1.5 AU-N/g

storage temp.

2-8°C

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General description

Protease from Bacillus sp. is extensively useful in pharmaceutical, leather, food and waste processing industry. It is widely used in manufacturing protein hydrolysates. Analysis of Bacillus protease stability and activity showed that the enzyme was active at all pH values from 5.0-11.0 with 100% activity at 8.0. Optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.

Application

Protease from Bacillus sp. has been used:
  • to evaluate protease inhibitor activity
  • in high yields in batch fermentation using a response surface methodology
  • in a study to investigate a biosensor to detect a protease biomarker from Bacillus licheniformis

Analysis Note

minimum activity 1.5 AU-N/G solid

Legal Information

Protamex is a registered trademark of Novozymes Corp.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hypocholesterolemic effect of compounded freshwater clam protein hydrolysate and Gracilaria
Lin, Y-H, et al
Food Chemistry, 123(2), 395-399 (2010)
Huiwang Wu et al.
The Analyst, 137(20), 4829-4833 (2012-08-30)
A simple, selective and sensitive electrochemical biosensor has been developed to detect protease biomarker from Bacillus licheniformis, a recognized model of the biochemical warfare agent Bacillus anthracis. In this assay, the biosensor is constructed using a d-amine acid containing substrate
Trypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial Origin
<BIG>Ali PPM, et al.</BIG>
Applied Biochemistry and Biotechnology, 167-178 (2014)
Anum Khan et al.
Molecular biology reports, 46(4), 4565-4580 (2019-06-28)
Polysaccharides including resistant starch are categorized as dietary fiber and are used as an important prebiotic. Similar to soluble fibers, resistant starch also has a number of physiological effects that have been shown to be beneficial for health. Starch hydrolyzing
Sumant Puri et al.
Current microbiology, 44(4), 286-290 (2002-03-23)
High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be

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