P2714

Protease Inhibitor Cocktail

lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder

• Synonym(s): Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: Protease Inhibitor Cocktail powder, for general use, lyophilized powder
• biological source: bovine lung
• Quality Level: 300
• form: lyophilized powder
• storage temp.: −20°C

Description

General description

The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.

Specificity

Inhibits serine, cysteine, and metallo-proteases.

Application

Protease Inhibitor Cocktail powder has been used as a component:

• in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
• in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
• in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
• in 0.1 M NaCl solution to wash and collect skin secretions of frog

Biochem/physiol Actions

The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Components

This mixture contains individual components, including:

AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM

Each component has specific inhibitory properties.

Caution

The lyophilized powder is stable for 4 years when stored at -20°C.

Quantity

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Preparation Note

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable