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P5538

Sigma-Aldrich

Phosphoglucose Isomerase from Bacillus stearothermophilus

lyophilized powder, 300-1,000 units/mg protein

Synonym(s):

D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

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25 ML
$391.00

$391.00


Estimated to ship onMarch 21, 2025


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25 ML
$391.00

About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

$391.00


Estimated to ship onMarch 21, 2025


Request a Bulk Order

form

lyophilized powder

Quality Level

specific activity

300-1,000 units/mg protein

mol wt

189 kDa

composition

Protein, ≥60% biuret

storage temp.

−20°C

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P9544P5381P2783
form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

mol wt

189 kDa

mol wt

-

mol wt

tetramer 119,500 Da±600

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ≥60% biuret

composition

protein, 70-100% biuret

composition

-

composition

Protein, ≥20%

General description

The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa.[1] Optimum pH is found to be between 9-10 and the isoelectric point is 4.2.

Application

Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus.
The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti.[2]

Biochem/physiol Actions

Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Unit Definition

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30 °C.

Physical form

lyophilized powder containing Tris buffer

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    A Arias et al.
    Journal of bacteriology, 137(1), 409-414 (1979-01-01)
    A mutant strain of complex phenotype was selected in Rhizobium meliloti after nitrosoguanidine mutagenesis. It failed to grow on mannitol, sorbitol, fructose, mannose, ribose, arabitol, or xylose, but grew on glucose, maltose, gluconate, L-arabinose, and many other carbohydrates. Assay showed
    The Kinetics and Mechanism of a Reaction Catalyzed by Bacillus stearothermophilus Phosphoglucose Isomerase.
    Widjaja A, et al.
    Journal of Fermentation and Bioengineering, 86(3), 324-331 (1998)
    Natsuko Miura et al.
    Eukaryotic cell, 11(8), 1075-1082 (2012-07-04)
    Glycolytic enzymes are cytosolic proteins, but they also play important extracellular roles in cell-cell communication and infection. We used Saccharomyces cerevisiae to analyze the secretory pathway of some of these enzymes, including enolase, phosphoglucose isomerase, triose phosphate isomerase, and fructose
    Prashant Warang et al.
    International journal of hematology, 96(2), 263-267 (2012-07-12)
    Homozygous glucose phosphate isomerase (GPI) deficiency is one of the most important erythroenzymopathies causing hereditary non-spherocytic hemolytic anemia (HNSHA). We report an Indian patient with HNSHA showing 85 % reduction in GPI activity resulting from a homozygous missense replacement g.1459C
    Yuki Usui et al.
    Microbial cell factories, 11, 87-87 (2012-06-23)
    It has long been recognized that analyzing the behaviour of the complex intracellular biological networks is important for breeding industrially useful microorganisms. However, because of the complexity of these biological networks, it is currently not possible to obtain all the

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