PPC2020

Protease and Phosphatase Inhibitor Cocktail

ReadyShield^® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidases, L-isozymes of alkaline phosphatases, non-freezing solution

• Synonym(s): Protease and phosphatase inhibitor cocktail, inhibitor cocktail
• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• product name: ReadyShield^® Protease and Phosphatase Inhibitor Cocktail, non-freezing solution
• Quality Level: 200
• form: ready-to-use solution
• packaging: pkg of 1 mL (in amber polypropylene vial); pkg of 5 mL (in amber polypropylene vial)
• storage temp.: −20°C

Description

General description

Protease and Phosphatase Inhibitor Cocktail has been optimized and tested for mammalian cellular and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, acid proteases, aminopeptidases, L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases.

Protease and Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

The cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield^® PPC2020 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail PPC1010

Features and Benefits

• Does not freeze in storage for quick, convenient use.
• For broad ranging inhibition of both proteases and phosphatases.
• Suggested as 100 X solution.
• Reclosable screw-top vials.
• Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable