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S8015

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

grade

for molecular biology

form

powder blend

pH

6.9-7.1 (20-25 °C, 20 ×)

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization. SSC buffer powder should be reconstituted to the final indicated volume using molecular biology grade water.

Application

Suitable for use in washing buffer for Northern and Southern hybridization procedures.

Features and Benefits

  • Easy to reconstitute powder
  • Multi-purpose use in hybridizations

Components

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

related product

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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