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SAB4200572

Sigma-Aldrich

Anti-Neuron-Specific Enolase (NSE) antibody, Mouse monoclonal

clone NSE-P2, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-2-phospho-D-glycerate hydro-lyase, Monoclonal Anti-2-phospho-D-glycerate hydrolyase, Monoclonal Anti-Enolase 2 (gamma, neuronal), Monoclonal Anti-NSE, Monoclonal Anti-Neural enolase, Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse, Monoclonal Anti-gamma-enolase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NSE-P2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~47 kDa

species reactivity

human, rat

concentration

~1 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.
western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ENO2(2026)

General description

Monoclonal Anti-Neuron-specific Enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neuron-specific enolase (NSE) is present in the brain especially in the neuroendocrine cells and neurons. It is a cytosolic enzyme, that exists as a dimer and belongs to the family of enolase enzymes. NSE gene is mapped to human chromosome12p13.31.

Specificity

Monoclonal Anti-Neuron-specific Enolase (NSE) recognizes human and rat NSE.

Immunogen

synthetic peptide corresponding to an internal sequence of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse may be used in:
  • immunoblotting
  • enzyme-linked immunosorbent assay (ELISA)
  • immunohistochemistry

Biochem/physiol Actions

Neuron-specific enolase (NSE) catalyzes the production of phosphoenolpyruvate from 2-phospho-D-glycerate in the glycolytic pathway. NSE is a specific neurobiochemical marker of brain damage after traumatic brain injury, hypoxic encephalopathy and stroke. Moreover, NSE has been suggested to be a general circulating marker for neuroendocrine tumors. Interestingly, high levels of NSE were also reported in breast cancer upon exposure to the environmental pollutants arsenite and cadmium.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Circulating biomarkers in gastroenteropancreatic neuroendocrine tumours
Oberg K
Endocrine-Related Cancer, 18(S1), S17-S25 (2011)
New insights into the role of neuron-specific enolase in tic disorders
Hao J, et al.
Neurological Sciences, 40(6), 1167-1172 (2019)
Linkage to peroxisome proliferator-activated receptor-gamma in SAMP1/YitFc mice and in human Crohn?s disease
Sugawara K, et al.
Gastroenterology, 128(2), 351-360 (2005)
Neurological biomarkers in the perioperative period
Cata JP, et al.
British Journal of Anaesthesia, 107(6), 844-858 (2011)
Arsenic, cadmium and neuron specific enolase (ENO2, gamma-enolase) expression in breast cancer
Soh MA, et al.
Cancer Cell International, 11(1), 41-41 (2011)

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