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1X Phosphate-Buffered Saline (PBS) Recipe Calculator

Preparing PBS 1X by Volume

Phosphate-buffered saline (PBS) is an isotonic solution that is used in many biological research applications. To make 1 L of PBS, add 100 mL of 10X PBS to 900 mL of water. This PBS recipe contains 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4.

This calculator enables the accurate preparation of this 1X PBS wash buffer for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed to make this common buffer used for Western blotting and other immunoassays.

1X PBS Solution Concentrations:

  • NaCl: 137 mM
  • KCl: 2.7 mM
  • Na2HPO4: 10 mM
  • KH2PO4: 1.8 mM

PBS Recipe Calculator

1 L Recipe Desired Volume: mL    
100 mL of 10X PBS Stock Solution   mL of 10X PBS Stock Solution
900 mL of Milli-Q® Water   mL of Milli-Q® Water
  The resultant 1X PBS has a final concentration of 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl; pH 7.4.
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