Stock Components for Western Blot Stripping Solutions
This calculator enables the accurate preparation of stock components for the detergent-based Western blot stripping solution for any volume that you need. Input your desired volume and click the CALCULATE button. The table will populate with the amounts of each component needed.
0.5 M Tris-HCl pH 6.8 — 100 mL Recipe | Desired Volume: mL |
6.06 g of Tris Base (FW 121.1) (93362) | g of Tris Base |
60 mL of Milli-Q® Water | mL of Milli-Q® Water |
Adjust to pH 6.8 with 12 N HCl (H1758) | Adjust to pH 6.8 with 12 N HCl |
Adjust final volume to 100 mL with Milli-Q® Water | Adjust final volume to mL with Milli-Q® Water |
10% SDS — 100 mL Recipe | Desired Volume: mL |
10 g of SDS (L3771) | g of SDS |
Adjust final volume to 100 mL with Milli-Q® Water | Adjust final volume to mL with Milli-Q® Water |
Dissolve by gently stirring; slowly adjust to final volume with Milli-Q® water. |
OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS
Stock Solutions
- 4X SDS Sample Loading Buffer
- 10X Phosphate-Buffered Saline (PBS)
- 10X Tris-Buffered Saline (TBS) Stock Solution
Wash & Blocking Buffers
- 1X Tris-Buffered Saline (TBS) Working Solution
- 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
- 1X Phosphate-Buffered Saline (PBS) Recipe Calculator
- 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
- Non-Fat Dry Milk (NFDM) Blocking Buffer
- Bovine Serum Albumin (BSA) Blocking Buffer
Stripping Solutions
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