추천 제품
생물학적 소스
chicken
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
rat
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
rat ... Aqp9(65054)
일반 설명
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane.
A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
특이성
Aquaporin 9. The immunogen peptide is unique to Aquaporin-9 without significant homology to any other Aquaporins.
Rat. The immunogen peptide has 94% conservation with mouse and 72% conservation with the human Aquaporin-9. Species reactivity has not been confirmed.
Rat. The immunogen peptide has 94% conservation with mouse and 72% conservation with the human Aquaporin-9. Species reactivity has not been confirmed.
면역원
An 18 AA synthetic peptide within the C-terminal domain of rat Aquaporin-9 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.
애플리케이션
Detect Aquaporin 9 using this Anti-Aquaporin 9 Antibody validated for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1-10 μg/mL using ECL
ELISA: 1:100,000 using 50-100 ng of control peptide per well.
Immunohisto/cytochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues. Adherent cells can be fixed with 50% methanol-50% acetone or 1% paraformaldehyde.
Optimal working dilutions must be determined by end user.
ELISA: 1:100,000 using 50-100 ng of control peptide per well.
Immunohisto/cytochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues. Adherent cells can be fixed with 50% methanol-50% acetone or 1% paraformaldehyde.
Optimal working dilutions must be determined by end user.
물리적 형태
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
저장 및 안정성
Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Agmatine Attenuates Brain Edema and Apoptotic Cell Death after Traumatic Brain Injury.
Kim, JY; Lee, YW; Kim, JH; Lee, WT; Park, KA; Lee, JE
Journal of Korean Medical Science null
Elizabeth Kimball et al.
PloS one, 16(2), e0244123-e0244123 (2021-02-03)
To study aquaporin channel expression in astrocytes of the mouse optic nerve (ON) and the response to IOP elevation in mice lacking aquaporin 4 (AQP4 null). C57BL/6 (B6) and AQP4 null mice were exposed to bead-induced IOP elevation for 3
Elizabeth C Kimball et al.
PloS one, 17(6), e0268541-e0268541 (2022-06-17)
Aquaporin 4 is absent from astrocytes in the rodent optic nerve head, despite high expression in the retina and myelinated optic nerve. The purpose of this study was to quantify regional aquaporin channel expression in astrocytes of the porcine and
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