추천 제품
제품명
Anti-Tubulin Antibody, Detyrosinated, Chemicon®, from rabbit
생물학적 소스
rabbit
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
rat, mouse, sea urchin, human, plant
반응하면 안 됨
Saccharomyces cerevisiae
종 반응성(상동성에 의해 예측)
mammals
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... TUBA1A(7846)
일반 설명
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
특이성
Expected to cross-react with mammals, sea urchin, plants, not yeast (Paturle-Lafanechere, L., 1991).
The antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005).
면역원
Peptide corresponding to the C-terminal of Glu tubulin.
애플리케이션
Anti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) and WB.
Immunohistochemistry:
A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution.
Optimal working dilutions must be determined by the end user.
A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
품질
Routinely evaluated by Western Blot on L6 lysate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate.
Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate.
표적 설명
50 kDa
물리적 형태
Affinity purified immunoglobulin
Purified rabbit polyclonal in buffer containing liquid PBS.
저장 및 안정성
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
Hela Whole Cell Lysate.
Hela Whole Cell Lysate.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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