MABS27

Anti-Diglycyl Lysine (Clone GX41) Antibody

clone GX41, from mouse

• 동의어(들): Diglycyl Lysine
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: GX41, monoclonal
• 종 반응성: human, mouse, rat
• 종 반응성(상동성에 의해 예측): all
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Anti-Diglycyl Lysine has been shown to specifically detect peptides derived from the ubiquitinated portion of proteins. Diglycyl Lysine, clone GX41 is thought to find diglycyl-lysine moieties that are created and left behind at places of ubiquitination after digestion with trypsin has occurred.

특이성

Antibody recognizes lysine residues modified by diglycine, a residue left at sites of ubiquitination after trypsin digestions.

Broad species cross-reactivity expected based on 100% sequence homology.

면역원

Linear peptide corresponding to Diglycyl Lysine.

애플리케이션

Anti-Diglycyl Lysine (Clone GX41) Antibody detects level of Diglycyl Lysine (Clone GX41) & has been published & validated for use in WB & IP.

Immunoprecipitation Analysis: 10 µg of Anti-Diglycyl Lysine immunoprecipitated peptides containing Diglycyl Lysine modification from isolated ubiquitinated proteins which were obtained from a human cell line.

Mass Spectrometry Analysis: A previous lot was used by an independent a laboratory in Mass Spec. Image courtesy of Samie Jaffrey, Cornell Medical College.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

품질

Evaluated by Western Blot in BSA protein and Diglycine modified BSA.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Diglycyl Lysine on 10 µg of BSA protein and Diglycine modified BSA.

표적 설명

Molecular weight can vary depending on the size of the protein or peptides containing Diglycyl modified lysine residues.

물리적 형태

Format: Purified

Protein G

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
BSA protein and Diglycine modified BSA

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable