추천 제품
생물학적 소스
goat
Quality Level
결합
alkaline phosphatase conjugate
항체 형태
IgG fraction of antiserum
항체 생산 유형
secondary antibodies
클론
polyclonal
종 반응성
rabbit
기술
direct ELISA: 1:20,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:100,000
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
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일반 설명
IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains, held together by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.
특이성
Goat polyclonal anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody reacts with rabbit IgG in vitro, in rabbit serum and biological fluids.
면역원
pooled normal rabbit serum
애플리케이션
Anti-Rabbit IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat has been used in western blotting and enzyme-linked immunosorbent assay (ELISA).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
생화학적/생리학적 작용
IgG1 is most abundant and its deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region contributes for the development of therapeutic antibody.
물리적 형태
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide
법적 정보
CDP-Star is a registered trademark of Tropix, Inc.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
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