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Merck
모든 사진(5)

주요 문서

A6535

Sigma-Aldrich

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

동의어(들):

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

유형

Grade VII

Quality Level

양식

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

구성

Protein, 25-60%

solubility

H2O: soluble 1.0 mg/mL

응용 분야

diagnostic assay manufacturing

외래 활성

Acid Phosphatase ≤2% of base activity

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

생화학적/생리학적 작용

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

포장

Sold on the basis of ATPase units.

기타 정보

This grade has a low ATPase/ADPase ratio.

단위 정의

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

물리적 형태

Partially purified, lyophilized powder containing potassium succinate buffer salts.

제조 메모

Derived from red potato

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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